Insertion junction: LMJ.RY0402.219104_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.219104
Chromosome:chromosome 6
Location:7917069
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g303350 FAL9 Conserved protein of unknown function intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACCCAGCCACACGCCCCTCCCCATCCC
Internal bar code:GAACGGGCGGTACGGAGTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:377
LEAP-Seq percent confirming:87.4106
LEAP-Seq n confirming:611
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGTGCACAATGAACAAC
Suggested primer 2:CTCTGCCACCTATCACAGCA