| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219118 |
| Chromosome: | chromosome 3 |
| Location: | 7290574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207000 | (1 of 1) PTHR34543:SF1 - ABSCISIC ACID (ABA)-DEFICIENT 4 PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGATGATAATGGAGCCGGACTCGCTCT |
| Internal bar code: | CGTTTGGATGCGTGGTCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 99.6704 |
| LEAP-Seq n confirming: | 5443 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTCGCATCATCCGTTAC |
| Suggested primer 2: | AAGACCTCGGGCTTCTTCTC |