| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219130 |
| Chromosome: | chromosome 11 |
| Location: | 382846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467577 | (1 of 2) PF00319 - SRF-type transcription factor (DNA-binding and dimerisation domain) (SRF-TF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGCAGCCTGTTGAACAGCAACCAAGGC |
| Internal bar code: | TGAGGGGATTAAGTAGATCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 53.6336 |
| LEAP-Seq n confirming: | 1572 |
| LEAP-Seq n nonconfirming: | 1359 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAATCACGGCTGTTAGA |
| Suggested primer 2: | TGTCCTGACGAACGCTACAC |