| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219164 |
| Chromosome: | chromosome 12 |
| Location: | 6121813 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535950 | NUOS1 | (1 of 1) K03934 - NADH dehydrogenase (ubiquinone) Fe-S protein 1 (NDUFS1); NADH:ubiquinone oxidoreductase 76 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCATTTATCAACTAGTGCCTCGGCTTA |
| Internal bar code: | TGCGGTTACGGCTATGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.5851 |
| LEAP-Seq n confirming: | 12481 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAATCAAGCAAGAAGGGC |
| Suggested primer 2: | CACCTCCACCATCTCCAACT |