| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219181 |
| Chromosome: | chromosome 17 |
| Location: | 4015468 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728976 | (1 of 1) IPR000104//IPR001254//IPR009003 - Antifreeze protein, type I // Serine proteases, trypsin domain // Peptidase S1, PA clan | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGTCTCCACGAAATTGTCTCCGTCCAG |
| Internal bar code: | GGGGACAGGTCCGTTCGTCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 99.1725 |
| LEAP-Seq n confirming: | 6112 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCAACTAACGGAGTAAC |
| Suggested primer 2: | CATCAGTAAATGTGCGGTGG |