| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219195 |
| Chromosome: | chromosome 17 |
| Location: | 4469070 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732350 | GOX4 | Glyoxal oxidase 4; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTTTACTTAGTTGTGCTGTGCGTTTGC |
| Internal bar code: | TCAGTGCGGCTAGGCAACACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 99.2958 |
| LEAP-Seq n confirming: | 1833 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACGGAACAATTCAGCC |
| Suggested primer 2: | AAGTCATGGGATTGCTGGAC |