| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219195 |
| Chromosome: | chromosome 17 |
| Location: | 4469134 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732350 | GOX4 | Glyoxal oxidase 4; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGCTGTACGACCCGCCCACAAACAA |
| Internal bar code: | TTTAGGATGGTTCGGGCATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 99.3365 |
| LEAP-Seq n confirming: | 1647 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTCATTCGCTGTATCA |
| Suggested primer 2: | AAGTCATGGGATTGCTGGAC |