| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219196 |
| Chromosome: | chromosome 12 |
| Location: | 1670296 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486000 | (1 of 102) PF01753 - MYND finger (zf-MYND) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGTCCTCAGCGGGCGGGCCGCACACA |
| Internal bar code: | AGCGGTTGACGCACGGCCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 61.165 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTTCAGACTCGTAGGGC |
| Suggested primer 2: | CCAGCGTATTCTCACAGCAA |