| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219196 |
| Chromosome: | chromosome 12 |
| Location: | 1670297 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486000 | (1 of 102) PF01753 - MYND finger (zf-MYND) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCGCCCGCACAGCAACCAGGTACGCCA |
| Internal bar code: | TTTCGCGGGTTCCAACAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1123 |
| LEAP-Seq percent confirming: | 99.6429 |
| LEAP-Seq n confirming: | 279 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTTCAGACTCGTAGGGC |
| Suggested primer 2: | CCAGCGTATTCTCACAGCAA |