| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219202 |
| Chromosome: | chromosome 4 |
| Location: | 1286294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214850 | (1 of 1) IPR000104//IPR000253 - Antifreeze protein, type I // Forkhead-associated (FHA) domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATGCGCTGGGCACTTGCCTATGACGCA |
| Internal bar code: | CTATTGGGCGTGGCAGACAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 98.6882 |
| LEAP-Seq n confirming: | 5191 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGTCGGACACTTGGAC |
| Suggested primer 2: | GAAATCGCTACAGGCTACGG |