| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219209 |
| Chromosome: | chromosome 10 |
| Location: | 3417361 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444500 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCGAAGCCGCCTGCCAGGTTCACCAGC |
| Internal bar code: | CCGCGTACAATAGGTTCCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.4118 |
| LEAP-Seq n confirming: | 2028 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAGGAAGCTAATGGCTC |
| Suggested primer 2: | TTGCTGCTTGTACGTTTTCG |