| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219213 |
| Chromosome: | chromosome 16 |
| Location: | 7697426 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690767 | (1 of 3) PF02737 - 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (3HCDH_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGCCCCTCTCCGTGTGCAGTCGGGGC |
| Internal bar code: | AGATGGCCTCTTACATGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.5913 |
| LEAP-Seq n confirming: | 8529 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCATACGGCCATACTCAC |
| Suggested primer 2: | GTCTGTGTGCGAGGGGTAAT |