| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219255 |
| Chromosome: | chromosome 1 |
| Location: | 3162642 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCGCCTCGCACCCCAACCCCGTTTTT |
| Internal bar code: | GGACTGTGAAGAACAAACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 69.3694 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATGTCCGTGTCTATGC |
| Suggested primer 2: | GTAACACACACACCGCCAAC |