| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219279 |
| Chromosome: | chromosome 11 |
| Location: | 3423875 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480851 | RRA3 | (1 of 3) PTHR10994//PTHR10994:SF3 - RETICULON // ARABINOSYLTRANSFERASE; Reduced residual arabinose 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGATACCTATGATACAAATGATGCCGA |
| Internal bar code: | GATCGATGATGCGCGCGTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 99.2366 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCCTCTGTATCCACAA |
| Suggested primer 2: | GCACCTGAAGAACCTCTTGC |