Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.219282 |
Chromosome: | chromosome_12 |
Location: | 1560488 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g486800 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTGCTGCAATCTCGTGTTCACGGAGCTCT |
Internal bar code: | GCGTAGGAGCAGCACGAGAACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 786 |
LEAP-Seq percent confirming: | 99.5588 |
LEAP-Seq n confirming: | 2031 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGGGTTTGGGACGCTTAGT |
Suggested primer 2: | TGCTACTCCGCATGAACTTG |