Insertion junction: LMJ.RY0402.219282_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219282
Chromosome:chromosome 16
Location:4656889
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g687400 (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCACTTGTGGTCGTCAAGTACACCCCGC
Internal bar code:CACAGGCGCAGGCGTGGTAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:139
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGTGCTCCATAATCAGA
Suggested primer 2:CGTGTTTCTGGTGGATGTTG