Insertion junction: LMJ.RY0402.219286_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.219286
Chromosome:chromosome 6
Location:3571075
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278119 (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGCGGTGCATGAAGATGAAGTGTGCTT
Internal bar code:TCTTGCCGAGCAGACATTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:462
LEAP-Seq percent confirming:99.6956
LEAP-Seq n confirming:1310
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGAGTACTCGATCAGCG
Suggested primer 2:CGCACAGGCAAACTCATCTA