Insertion junction: LMJ.RY0402.219312_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219312
Chromosome:chromosome 1
Location:7867931
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g060347 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGACCCCTGCGCGCACTACCGTACACGC
Internal bar code:ATGGGGCCGAGGGGAAACGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:849
LEAP-Seq percent confirming:98.5208
LEAP-Seq n confirming:3863
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCCAGGATAGCGCGTAG
Suggested primer 2:AGTGCCAACGATACTGTCCC