Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.219312 |
Chromosome: | chromosome 1 |
Location: | 7867931 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g060347 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACCCCTGCGCGCACTACCGTACACGC |
Internal bar code: | ATGGGGCCGAGGGGAAACGCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 849 |
LEAP-Seq percent confirming: | 98.5208 |
LEAP-Seq n confirming: | 3863 |
LEAP-Seq n nonconfirming: | 58 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTCCAGGATAGCGCGTAG |
Suggested primer 2: | AGTGCCAACGATACTGTCCC |