Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.219316 |
Chromosome: | chromosome_3 |
Location: | 6482884 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g194900 | CYA4 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGCCGTCGAACGGACGCTTGCACGGGAAC |
Internal bar code: | TTAATCGAACGGCTGTACATGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 111 |
LEAP-Seq percent confirming: | 99.6422 |
LEAP-Seq n confirming: | 1114 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGCTCTCTCTCTTTCGCTG |
Suggested primer 2: | GTGCAGGTGTCTGTGTGCTT |