| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219336 |
| Chromosome: | chromosome 7 |
| Location: | 4930312 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346450 | (1 of 1) PTHR11780//PTHR11780:SF12 - NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1 // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGATATAAAGTCCAGGCTCAGCGCTC |
| Internal bar code: | TTTGGTTCGACCTTCCAGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 23.7477 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 411 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGGCAGTATTGTTGGC |
| Suggested primer 2: | TGCTTACAATCCAATCTGCG |