| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219342 |
| Chromosome: | chromosome 1 |
| Location: | 2409421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGGCTGGTATAGATCCGCGCTGGCCC |
| Internal bar code: | GCTAATATCTGTATGCTAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 98.0695 |
| LEAP-Seq n confirming: | 2032 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGAGCTACCGCATTTG |
| Suggested primer 2: | AACACTTGGGTACCGCTTTG |