| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219359 |
| Chromosome: | chromosome 12 |
| Location: | 1246737 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489400 | PTB7 | (1 of 9) K14640 - solute carrier family 20 (sodium-dependent phosphate transporter) (SLC20A, PIT); Sodium/phosphate symporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGATGATGACCTGTGGAGTGGGGTCAGG |
| Internal bar code: | CGGGTGCCACAATCACCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.0345 |
| LEAP-Seq n confirming: | 2667 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGAAGACAGGAGGGGT |
| Suggested primer 2: | CATCCGGAACCAGGAAGTAA |