| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219386 |
| Chromosome: | chromosome 14 |
| Location: | 2907962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627500 | (1 of 1) PTHR18937//PTHR18937:SF259 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACGCACCTCGCCCTGCCGCCAACACAC |
| Internal bar code: | AAGCTTATCAACGTCCACCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 97.6676 |
| LEAP-Seq n confirming: | 2010 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTCATCCTCCTCATCC |
| Suggested primer 2: | GTGTGTGTGTGTGTGTGCGT |