| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219393 |
| Chromosome: | chromosome 8 |
| Location: | 4836276 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCCTGAGACCGTTACGGTATGTGTGTG |
| Internal bar code: | TTTAGCAGACTTGCACGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 99.562 |
| LEAP-Seq n confirming: | 10912 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTACACCACCAAACTCGC |
| Suggested primer 2: | CACTGCTACTGCCGCTACTG |