| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219393 |
| Chromosome: | chromosome 17 |
| Location: | 1704428 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708750 | NSG1,CSE21 | Nitrogen-starvation/gametogenesis expressed protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGTACTTATAAGCAGATGCAATGTAAC |
| Internal bar code: | ATTCGGGGAGCTCTCTACCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1158 |
| LEAP-Seq percent confirming: | 98.9326 |
| LEAP-Seq n confirming: | 1761 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGGTTCAAGGGTCACG |
| Suggested primer 2: | CACAGGGATCTGGTCTGGTT |