Insertion junction: LMJ.RY0402.219403_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.219403
Chromosome:chromosome 9
Location:5677862
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g401367 (1 of 1) PTHR15189:SF7 - BRCA1-A COMPLEX SUBUNIT BRE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACTCACCCATCCTCCCTCCCGCGGTCC
Internal bar code:CCGACGAGAACGGGTCCAGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:37
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTTCCTTCCTTTCCCACT
Suggested primer 2:GTCCTCTTCCTCCTCCTGCT