| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219407 |
| Chromosome: | chromosome 3 |
| Location: | 5665549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187500 | (1 of 1) PTHR23339:SF64 - CYCLIN-DEPENDENT KINASE INHIBITOR 3; Dual specificity phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTCCCGTGAGTGAATGCATGACTGGTG |
| Internal bar code: | CTCAAGTGCACACTCGCTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.785 |
| LEAP-Seq n confirming: | 2785 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACATGTACTCAACGCC |
| Suggested primer 2: | CACCGCACCCTGATTAAAGT |