| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219417 |
| Chromosome: | chromosome 6 |
| Location: | 8116986 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304850 | (1 of 1) PF04424 - Protein of unknown function (DUF544) (DUF544) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTGCATGTATGTATGTGATTTCAATAT |
| Internal bar code: | GGGAACTTCGCTTCCGAGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 99.7144 |
| LEAP-Seq n confirming: | 7681 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCGACCTTATCAACTT |
| Suggested primer 2: | GCTTGAAGGGTTTGAAGTCG |