Insertion junction: LMJ.RY0402.219447_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.219447
Chromosome:chromosome 16
Location:4030119
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g670200 Microtubule-associated protein cysteine-rich PDZ-binding protein; (1 of 2) PTHR11805:SF1 - CYSTEINE-RICH PDZ-BINDING PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGAAACAGCCCCGCCCACGGTCAAAAC
Internal bar code:TGGGTAAGAGTGATTAAAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1020
LEAP-Seq percent confirming:99.4158
LEAP-Seq n confirming:2042
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGTTAATGGGCTGTCAT
Suggested primer 2:AATGCAAGACGTGCAAACAG