| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219448 |
| Chromosome: | chromosome 13 |
| Location: | 1889266 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575776 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGAGCGCCATCCGTCGCTGCCGCCGCC |
| Internal bar code: | CTGGGGGATGCACGGACTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.139 |
| LEAP-Seq n confirming: | 6218 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGTGTGCGTGTTTGAG |
| Suggested primer 2: | TTGTGGTGACTGATCGGTGT |