| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219457 |
| Chromosome: | chromosome 1 |
| Location: | 4279195 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028713 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGTGCAACACTTAGTGGTCGGCCTTTAG |
| Internal bar code: | CTAACTAACGGGAGTTGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 99.565 |
| LEAP-Seq n confirming: | 3662 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGACACAACCTCCCGATT |
| Suggested primer 2: | ACCGAATACCCGCTAGTCCT |