| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219477 |
| Chromosome: | chromosome 12 |
| Location: | 3131966 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499700 | MFT3,MAE3 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF); MATE efflux family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGGGATGAGGTGGTAAAGCTTCGAAC |
| Internal bar code: | GTCACGAGGATTCGGGGCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1111 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 15484 |
| LEAP-Seq n nonconfirming: | 196 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGTCGCGAAACTGTTA |
| Suggested primer 2: | GTGTTCGGGACGATGAGTTT |