| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219497 |
| Chromosome: | chromosome 12 |
| Location: | 7840416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553100 | (1 of 1) PF16529 - WD40 region of Ge1, enhancer of mRNA-decapping protein (Ge1_WD40) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGACGGCGACTATGATGGCGGCGGCG |
| Internal bar code: | GCAAAACCGCGGGCATAGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 99.6511 |
| LEAP-Seq n confirming: | 1428 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCACTTCAGCCTCCTC |
| Suggested primer 2: | GTGAGCAGTGTGCATGTGTG |