Insertion junction: LMJ.RY0402.219503_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.219503
Chromosome:chromosome_11
Location:2546100
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g476050 ODA2,DHC15,DYHG Flagellar outer arm dynein heavy chain gamma sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTTACCAAGTCAGGGCATAATCATACACAG
Internal bar code:TTTAATCCGATGATTCGGATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:754
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGGACTTTGAGAGCCTG
Suggested primer 2:CGGAGTGATAGTGCTCGTCA