Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.219535 |
Chromosome: | chromosome_6 |
Location: | 2984816 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g273400 | TMC2 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCCCACATACACCATGCAGCCCAACAGCA |
Internal bar code: | GATGAGGCACGCTGTAACGCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 768 |
LEAP-Seq percent confirming: | 99.3741 |
LEAP-Seq n confirming: | 13972 |
LEAP-Seq n nonconfirming: | 88 |
LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTCAACAGCTTTGGTGTCA |
Suggested primer 2: | TGGACTTGGGCAAATAGGAC |