| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219535 |
| Chromosome: | chromosome_14 |
| Location: | 3194909 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCTGCCGGAGCCAACCAACATTACTTCT |
| Internal bar code: | TAAGTTCTGAATGAGCACACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 82.0513 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTTGGTGGTATGGAGGC |
| Suggested primer 2: | TTGCTGCTGATGCTGCTACT |