| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219540 |
| Chromosome: | chromosome 12 |
| Location: | 5521636 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531200 | FOX2 | Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGCGGGTGGCATCACGCTGCGCACC |
| Internal bar code: | TATTTGCAGTCACGTTGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 98.6528 |
| LEAP-Seq n confirming: | 6444 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTTCCTACTTGCTTCC |
| Suggested primer 2: | TATCGAGTGTTGGTCGCAAG |