| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219543 |
| Chromosome: | chromosome 11 |
| Location: | 2031893 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468400 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGGCCTGTCCTCCGACCGCATCATGGC |
| Internal bar code: | AATTCCGTTTCTAATTTCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 97.4537 |
| LEAP-Seq n confirming: | 421 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACGGCAACACAGTCATA |
| Suggested primer 2: | AAGGAGATCACGGTGGTGAC |