| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219548 |
| Chromosome: | chromosome 17 |
| Location: | 1651109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708100 | (1 of 3) 2.4.2.8 - Hypoxanthine phosphoribosyltransferase / Transphosphoribosidase; Similar to Hypoxanthine-Guanine | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTAGCATTACGCGGTAGACTGCCGCG |
| Internal bar code: | ATTTGATGGCCAGTTAGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 99.1909 |
| LEAP-Seq n confirming: | 4781 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGTGTTGCCCTTCATA |
| Suggested primer 2: | GACTGGACTTCGACGAGGAG |