| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219551 |
| Chromosome: | chromosome 3 |
| Location: | 5927615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190150 | CGL1 | (1 of 2) PF11833 - Protein of unknown function (DUF3353) (DUF3353); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGAGTTGCCCCTCCATGCTCGGCACGT |
| Internal bar code: | GAGTTTTTATCTAGGGGCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 99.6117 |
| LEAP-Seq n confirming: | 8466 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCGATGCATACAAGCAC |
| Suggested primer 2: | GACTCTGGCCACTTCCTCTG |