Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.219564 |
Chromosome: | chromosome_3 |
Location: | 1627526 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g152850 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGTGGCCAAGGTGGGGGCGGCGCTCCCT |
Internal bar code: | ACTGGCGGGAAGGGCAGGATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 405 |
LEAP-Seq percent confirming: | 97.4744 |
LEAP-Seq n confirming: | 3049 |
LEAP-Seq n nonconfirming: | 79 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAACAGGAAACAGGGAACA |
Suggested primer 2: | TTCTTCTCCGTGTACCTGGC |