| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219564 |
| Chromosome: | chromosome 3 |
| Location: | 1627526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152850 | PAM,PAM1 | (1 of 1) 1.14.17.3//4.3.2.5 - Peptidylglycine monooxygenase / Peptidylglycine alpha-amidating monooxygenase // Peptidylamidoglycolate lyase / Alpha-hydroxyglycine amidating dealkylase; bioactive peptide amidating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGGCCAAGGTGGGGGCGGCGCTCCCT |
| Internal bar code: | ACTGGCGGGAAGGGCAGGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 97.4744 |
| LEAP-Seq n confirming: | 3049 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACAGGAAACAGGGAACA |
| Suggested primer 2: | TTCTTCTCCGTGTACCTGGC |