| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219576 |
| Chromosome: | chromosome 2 |
| Location: | 4357515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099400 | ELG31 | Exostosin-like glycosyltransferase 31; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGAGCTGCAGGCCGCTAATCAGCAGCC |
| Internal bar code: | CAGCTCAAAAGAATGTAGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 97.884 |
| LEAP-Seq n confirming: | 7309 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTGTGCATCAACCAATG |
| Suggested primer 2: | TCCGGCTTATTATTTGCACC |