Insertion junction: LMJ.RY0402.219577_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.219577
Chromosome:chromosome 13
Location:3741846
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCTGCCCTCCACACACAGGTTCCGCGC
Internal bar code:GTGGCTAGCCTGATGACAGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0507099
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1971
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAACGAAATTGTGAGCCC
Suggested primer 2:GAGTGGCGCTACAGTGACAA