Insertion junction: LMJ.RY0402.219597_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219597
Chromosome:chromosome 7
Location:4099109
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g340750 EXN11 RNaseT, SDN2 homolog; (1 of 1) PTHR12801:SF69 - RNA EXONUCLEASE NEF-SP-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAAAGAGAAATATGCATCATGCATGGTGG
Internal bar code:GCACTCTGGCTCGACCCGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:884
LEAP-Seq percent confirming:94.472
LEAP-Seq n confirming:12168
LEAP-Seq n nonconfirming:712
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTCTGTGCACGTGTGTG
Suggested primer 2:GGCCATGCCCTAGATGAGTA