| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219605 |
| Chromosome: | chromosome 12 |
| Location: | 3578690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513650 | FAP342 | Flagellar Associated Protein 342 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGACCGCGCGCCGCCACCCCTCCTGAA |
| Internal bar code: | CAGTTCTGTTGTACTGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.5924 |
| LEAP-Seq n confirming: | 4887 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTGCTGATTGTGGAGCG |
| Suggested primer 2: | ACCTCCTCCACCACTTTCCT |