| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219625 |
| Chromosome: | chromosome 4 |
| Location: | 2594893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222650 | (1 of 18) PTHR19862//PTHR19862:SF14 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 48 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGGAAGCATTGTAAGCGCTGCCGCCGC |
| Internal bar code: | GCGGACGATAGAGCACAAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 81.4815 |
| LEAP-Seq n confirming: | 4466 |
| LEAP-Seq n nonconfirming: | 1015 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAACATCGCTCGGAAAGG |
| Suggested primer 2: | CACAGCCGAGCAGTTCATTA |