| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219628 |
| Chromosome: | chromosome 7 |
| Location: | 3537559 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336250 | (1 of 1) K13121 - protein FRA10AC1 (FRA10AC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTACATACTAGGCCTTTTCCAGCTCGG |
| Internal bar code: | CCGGTTATCGACTAGATTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCTTCAAGCTCAATTAC |
| Suggested primer 2: | CATTCATGTGTTGACCTCCG |