| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219637 |
| Chromosome: | chromosome 2 |
| Location: | 4633741 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101150 | (1 of 2) IPR000104//IPR000210//IPR011333 - Antifreeze protein, type I // BTB/POZ domain // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCCGCACAGGGCCCTGCGCCGCCGGCG |
| Internal bar code: | CGTGACGGTTGACGATGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.4521 |
| LEAP-Seq n confirming: | 4356 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTCCTTGGTTCCTTC |
| Suggested primer 2: | GGTGGTGGAGGTGTAATGCT |