| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219637 |
| Chromosome: | chromosome 2 |
| Location: | 4633746 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101150 | (1 of 2) IPR000104//IPR000210//IPR011333 - Antifreeze protein, type I // BTB/POZ domain // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACACCCCGCGATCGCAACGCCAGCCA |
| Internal bar code: | TTATTTCGATAGACATTACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 99.6709 |
| LEAP-Seq n confirming: | 17262 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTCCTTGGTTCCTTC |
| Suggested primer 2: | GGTGGTGGAGGTGTAATGCT |