| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219638 |
| Chromosome: | chromosome 12 |
| Location: | 3357526 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497350 | PPR13 | PentatricoPeptide Repeat protein 13 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCCAGCCCCAACACGCAGTTTCGCCTA |
| Internal bar code: | CTTGGATAGGCGTATGGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 90.5677 |
| LEAP-Seq n confirming: | 3111 |
| LEAP-Seq n nonconfirming: | 324 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCTTCTGCTGCTGAGTT |
| Suggested primer 2: | AGAATGGCCTCGTCATCATC |